Fig. 4

Immunophenotypic analysis of adaptive immunity (CD19⁺ B-cells; CD3⁺, CD3⁺CD4⁺ and CD3⁺CD8⁺ T-cells; T/B ratio and CD4⁺/CD8⁺ ratio in BALB/c mice immunized with the multicomponent LBSap vaccine, Leish-Tec® or Leishmune®. The x-axis displays the times at which the assays were conducted (before first vaccination [BV], 15 days after third saline [15^ASaline] or vaccination [15^AVac]] and 30 days after experimental L. infantum challenge [30^AChal]) in the different analyzed groups (n = 5 mice/group in two independent batches) (Control [□]; Leish-Tec® []; Leishmune® []; and LBSap [killed L. braziliensis vaccine plus saponin; ■]). The y-axis represents the mean (with standard deviations) of the frequency values in the lymphocytes gate. Connecting lines represent significant intra-group differences (P < 0.05) at the different time points. Inter-group differences at the same time point are marked by letters (C: Control group, LM: Leishmune® group, LB: LBSap group)