Table 1 Expression in NC of molecules involved in Wnt signaling pathway. Gene expression level determined by RT² Profiler PCR Arrays is given as 2^−∆CT, and by competitive microarray approach as a fold change, increase or decrease (↓) in relation to C2C12 myoblasts and myotubes. Data analysis was performed as described under Methods section

NM_007462

APC

Adenomatous polyposis coli

2.92 ± 0.40

NM_009733

AXIN1

Axin1

0.14 ± 0.06

NM_029933

BCL9

B-cell CLL/lymphoma 9

0.16 ± 0.03

NM_009771

BTRC

Beta-transducin repeat containing protein

0.63 ± 0.04

NM_023465

CTNNBIP1

Catenin beta interacting protein 1

1.59 ± 0.01

/2.1 (0.00)

NM_007631

CCND1

Cyclin D1

4.40 ± 0.62

/9.5 (0.00)*

NM_009829

CCND2

Cyclin D2

19.33 ± 1.41

5.1 (0.00)*/7.8 (0.00)*

NM_007632

CCND3

Cyclin D3

2.37 ± 0.64

2.1 (0.01)/↓1.9 (0.01)

NM_146087

CSNK1A1

Casein kinase 1, alpha 1

3.85 ± 1.01

/↓2.1 (0.00)

NM_139059

CSNK1D

Casein kinase 1, delta

7.36 ± 1.33

NM_007788

CSNK2A1

Casein kinase 2, alpha 1 polypeptide

9.55 ± 0.80

↓2.9 (0.00)/↓3.4 (0.00)

NM_013502

CTBP1

C-terminal binding protein 1

0.38 ± 0.12

NM_009980

CTBP2

C-terminal binding protein 2

5.26 ± 0.17

/2.2 (0.01)

NM_007614

CTNNB1

Catenin (cadherin associated protein), beta 1

2.03 ± 0.40

NM_172464

DAAM1

Dishevelled associated activator of morphogenesis 1

5.13 ± 0.47

NM_010091

DVL1

Dishevelled 1, dsh homolog (Drosophila)

0.48 ± 0.13

/↓2.2 (0.00)

NM_007888

DVL2

Dishevelled 2, dsh homolog (Drosophila)

0.09 ± 0.04

NM_177821

EP300

E1A binding protein p300

0.22 ± 0.06

NM_010234

FOS

v-FOS murine viral oncogene homolog

116.7 (0.00)*/27.2 (0.00)*

NM_008036

FOSB

FBJ murine viral oncogene homolog

6.0 (0.00)*/5.5 (0.00)*

NM_010235

FOSL1

Fos-like antigen 1

1.36 ± 0.13

↓3.1 (0.00)/2.1 (0.01)

NM_011356

FRZB

Frizzled-related protein

8.25 ± 2.13

21.9 (0.00)/14.8 (0.00)

NM_021457

FZD1

Frizzled homolog 1 (Drosophila)

1.36 ± 0.46

3.6 (0.00)/

NM_020510

FZD2

Frizzled homolog 2 (Drosophila)

1.20 ± 0.77

NM_021458

FZD3

Frizzled homolog 3 (Drosophila)

0.39 ± 0.04

NM_008055

FZD4

Frizzled homolog 4 (Drosophila)

0.35 ± 0.00

3.6 (0.01)/

NM_022721

FZD5

Frizzled homolog 5 (Drosophila)

1.97 ± 0.26

NM_008056

FZD6

Frizzled homolog 6 (Drosophila)

0.22 ± 0.11

NM_008057

FZD7

Frizzled homolog 7 (Drosophila)

0.07 ± 0.04

NM_008058

FZD8

Frizzled homolog 8 (Drosophila)

0.02 ± 0.01

3.4 (0.00)/2.4 (0.03)

NM_019827

GSK3B

Glycogen synthase kinase 3 beta

0.75 ± 0.02

NM_010591

JUN

Jun oncogene

7.68 ± 2.99

1.9 (0.00)/

NM_010592

JUND

Jun-D proto-oncogene

2.5 (0.02)*/2.1 (0.02)*

NM_032396

KREMEN1

Kringle containing transmembrane protein 1

8.56 ± 0.19

NM_010703

LEF1

Lymphoid enhancer binding factor 1

0.01 ± 0.003

↓3.7 (0.00)/↓2.4 (0.04)

NM_008513

LRP5

Low density lipoprotein receptor-related protein 5

1.43 ± 0.63

3.4 (0.00)/3.9 (0.00)

NM_008514

LRP6

Low density lipoprotein receptor-related protein 6

1.44 ± 0.09

NM_011945

MAP3K1

MEKK1, MAP kinase kinase kinase 1

4.9 (0.00)*/6.7 (0.00)*

NM_011948

MAP3K4

MEKK4, MAP kinase kinase kinase 4

2.1 (0.00)*/2.0 (0.00)

NM_009158

MAPK10

JNK3, Jun-N terminal kinase

8.8 (0.00)*/6.8 (0.00)*

NM_010849

MYC

Myelocytomatosis oncogene

0.88 ± 0.14

↓4.7 (0.00)*/↓2.9 (0.01)*

NM_027280

NKD1

Naked cuticle 1 homolog (Drosophila)

0.37 ± 0.04

42.3 (0.00)/27.8 (0.00)

NM_008702

NLK

Nemo-like kinase

0.38 ± 0.01

NM_019411

PPP2CA

Protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform

15.79 ± 0.81

NM_016891

PPP2R1A

Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform

10.09 ± 0.04

NM_009358

PPP2R5D

Protein phosphatase 2, regulatory subunit B (B56), delta isoform

0.75 ± 0.14

NM_008855

PRKCB1

Protein kinase C, beta 1

10.0 (0.01)*/6.7 (0.00)*

AK017901

PRKCE

Protein kinase C, epsilon

4.3 (0.01)*/4.4 (0.00)*

NM_008859

PRKCQ

Protein kinase C, theta

6.3 (0.00)*/4.6 (0.01)*

NM_008860

PRKCZ

Protein kinase C, zeta

14.6 (0.00)*/10.6 (0.00)*

NM_028116

PYGO1

Pygopus 1

0.38 ± 0.01

4.6 (0.00)/3.0 (0.01)

NM_133955

RHOU

Ras homolog gene family, member U

0.51 ± 0.16

/2.3 (0.00)

NM_029457

SENP2

SUMO/sentrin specific peptidase 2

1.88 ± 0.05

NM_013834

SFRP1

Secreted frizzled-related protein 1

0.09 ± 0.008

NM_009144

SFRP2

Secreted frizzled-related protein 2

0.02 ± 0.012

/↓5.4 (0.00)

NM_016687

SFRP4

Secreted frizzled-related protein 4

0.01 ± 0.003

NM_009332

TCF3

Transcription factor 7-like 1 (T-cell specific, HMG box)

1.31 ± 0.10

2.1 (0.02)/2.6 (0.00)

NM_009331

TCF7

Transcription factor 7, T-cell specific

0.46 ± 0.13

↓3.2 (0.00)/↓2.3 (0.01)

NM_011599

TLE1

Transducin-like enhancer of split 1

1.10 ± 0.27

NM_019725

TLE2

Transducin-like enhancer of split 2

0.005 ± 0.0022

NM_009506

VEGFC

Vascular endothelial growth factor C

5.3 (0.00)*/5.9 (0.00)*

NM_011915

WIF1

Wnt inhibitory factor 1

0.15 ± 0.02

6.4 (0.00)/2.5 (0.00)

NM_018865

WISP1

WNT1 inducible signaling pathway protein 1

2.22 ± 0.69

↓4.9 (0.00)/↓6.1 (0.00)

NM_021279

WNT1

Wingless-related MMTV integration site 1

0.003 ± 0.0011

NM_009519

WNT11

Wingless-related MMTV integration site 11

1.89 ± 0.22

6.5 (0.00)/4.1 (0.00)

NM_053116

WNT16

Wingless-related MMTV integration site 16

0.14 ± 0.01

5.6 (0.01)/3.3 (0.00)

NM_023653

WNT2

Wingless-related MMTV integration site 2

0.008 ± 0.0029

28.2 (0.00)*/21.9 (0.00)*

NM_009520

WNT2B

Wingless related MMTV integration site 2b

0.03 ± 0.001

NM_009521

WNT3

Wingless-related MMTV integration site 3

0.002 ± 0.0013

3.3 (0.01)/2.4 (0.00)

NM_009522

WNT3A

Wingless-related MMTV integration site 3A

0.004 ± 0.0022

NM_009523

WNT4

Wingless-related MMTV integration site 4

0.009 ± 0.0062

↓2.9 (0.00)/

NM_009524

WNT5A

Wingless-related MMTV integration site 5A

0.03 ± 0.011

4.8 (0.01)/3.0 (0.02)

NM_009525

WNT5B

Wingless-related MMTV integration site 5B

5.89 ± 2.90

9.9 (0.00)/7.7 (0.00)

NM_009526

WNT6

Wingless-related MMTV integration site 6

0.03 ± 0.008

↓2.8 (0.01)/

NM_139298

WNT9A

Wingless-type MMTV integration site 9A

0.01 ± 0.005

2.5 (0.00)/↓2.1 (0.00)