Occurrence and multilocus genotyping of Giardia intestinalis assemblage C and D in farmed raccoon dogs, Nyctereutes procyonoides, in China

Table 1 Primers used in the study, annealing temperatures used in the PCRs and expected sizes of the PCR products

Gene	Primer	Sequence (5′–3′)	Annealing temperature (°C)	Fragment length (bp)	Reference
tpi	F1	AAATIATGCCTGCTCGTCG	55	530	[3]
	R1	CAAACCTTITCCGCAAACC	55
	F2	CCCTTCATCGGIGGTAACTT	55
	R2	GTGGCCACCACICCCGTGCC	55
gdh	F1	TTCCGTRTYCAGTACAACTC	50	530	[3]
	R1	ACCTCGTTCTGRGTGGCGCA	50
	F2	ATGACYGAGCTYCAGAGGCACGT	65
	R2	GTGGCGCARGGCATGATGCA	65
bg	F1	AAGCCCGACGACCTCACCCGCAGTGC	50	511	[3]
	R1	GAGGCCGCCCTGGATCTTCGAGACGAC	50
	F2	GAACGAACGAGATCGAGGTCCG	60
	R2	CTCGACGAGCTTCGTGTT	60

ISSN: 1756-3305