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Table 1 Standard curve dilutions on each Ov-16-IgG4 ELISA plate. The mean and standard deviation obtained for each dilution and the arbitrary units assigned to each dilution are shown. The plates were read when the 1/8 dilution reached a 0.5 OD value. The 1/64 dilution was determined as the cut-off, hence samples with OD-values above this value were considered positive

From: Evaluation of onchocerciasis seroprevalence in Bioko Island (Equatorial Guinea) after years of disease control programmes

Standard curve dilutions Target mean (OD) Standard deviation Units
1/8 0.512 0.058 320
1/16 0.243 0.032 160
1/32 0.119 0.020 80
1/64 0.061 0.010 40
1/128 0.032 0.005 20
  1. The cut-off dilution and the corresponding OD value are marked in bold