Table 1 Standard curve dilutions on each Ov-16-IgG4 ELISA plate. The mean and standard deviation obtained for each dilution and the arbitrary units assigned to each dilution are shown. The plates were read when the 1/8 dilution reached a 0.5 OD value. The 1/64 dilution was determined as the cut-off, hence samples with OD-values above this value were considered positive
Standard curve dilutions | Target mean (OD) | Standard deviation | Units |
|---|---|---|---|
1/8 | 0.512 | 0.058 | 320 |
1/16 | 0.243 | 0.032 | 160 |
1/32 | 0.119 | 0.020 | 80 |
1/64 | 0.061 | 0.010 | 40 |
1/128 | 0.032 | 0.005 | 20 |