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Table 2 Real-time PCR on ticks that were exposed to rickettsiae by feeding on Rickettsia rickettsii-infected horses

From: Experimental infection of horses with Rickettsia rickettsii

Group Horse No. of positive ticks in real-time PCR/no. of tested ticks Total
   Unfed nymphs (fed as larvae on horses and molted to nymphs) Unfed adults (fed as larvae on horses, fed as nymphs on rabbits, and molted to adults) Unfed adults (fed as nymphs on horses and molted to adults) Fed adults (fed as nymphs on horses and fed as adults on rabbits)a Fed adults (fed as adults on horses)a  
Group 1b 1 2/90 (2.2 %)   2/56 (3.6 %) 0/83 (0) 12/51 (2.4 %) 16/280 (5.7 %)
2     0/70 (0) 0/29 (0) 0/99 (0)
Group 2b 3   0/7 (0)   1/189 (0.5 %) 0/21 (0) 1/217 (0.5 %)
4   1/178 (0.6 %)   0/69 (0) 0/22 (0) 1/269 (0.4 %)
Total   2/90 (2.2 %) 1/185 (0.5 %) 2/56 (3.6 %) 1/411 (0.2 %) 12/123 (9.8 %) 18/865 (2.1 %)
  1. aEngorged females collected from horses or rabbits were tested by real-time PCR after oviposition. Males recovered from horses or rabbits were tested soon after collection
  2. bRickettsial infection in Group 1 (horses 1 and 2) were via Rickettsia rickettsii-infected Amblyomma sculptum ticks, whereas Group 2 (horses 3 and 4) were inoculated intraperitoneally with macerated organs from R. rickettsii-infected guinea pigs