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Table 2 Comparison of storage methods, and factors important to the selection of the appropriate method

From: Maintenance of host DNA integrity in field-preserved mosquito (Diptera: Culicidae) blood meals for identification by DNA barcoding

Storage method Suitability for short-term field storage Suitability for long-term field storage Cost Operational concerns Additional requirements Conclusions
Filter paper Excellent Excellent Comparatively expensive: ~$500 per pack of 100 Cards holding DNA specimens require careful handling to protect from contamination during storage and transport. Supply of sterile instruments or ability to sterilize instruments used to macerate insect abdomen onto card. Effective for short- and long-term specimen storage. Substantial cost is a disadvantage.
EtOH Excellent Suitable Negligible: < $5 per liter Airline restrictions on ethanol in baggage, and transporting ethanol internationally. Tubes, or other containers to individualize blood meal specimens. Effective for short-term storage, and inexpensive, but potential transportation issues.
-20 °C Poor Poor Negligible Continued preservation required during transit. Availability of equipment. Access to electricity for freezers, or supply of dry ice or liquid nitrogen. Cold storage at -20 °C should be avoided, even when field conditions permit its use.
Silica Poor Poor Negligible Airtight containers needed to protect silica from moisture absorption. Tubes, or other containers to individualize blood meal specimens. Preservation of blood meal specimens is ineffective. The method should be avoided.