Fig. 1
Course of infection by L. amazonensis challenge (Josefa strain) in C57BL/6 mice from different sources. Leishmania amazonensis were cultured on M199 Medium. C57Bl/6-UNICAMP (a, b), C57Bl/6-FIOCRUZ (c, d), C57Bl/6-UFRJ (e, f) and C57Bl/6-UFF (g, h) were infected in the footpads with 5 × 105 stationary-phase promastigotes of L. amazonensis by subcutaneous route. Lesion sizes were measured at the indicated days and are expressed as the difference in thickness between non-infected and infected footpads (a, c, e, g). Parasite load was measured at the end of the experiment and expressed as the mean number of parasites in each footpad (b, d, f, h). The data (means ± standard deviations; n = 4–5) are representative of two (a, b) and three (c, d, e, f, g, h) independent experiments producing the same result profile. *P ≤ 0.05 in comparison to peak of infection (a, 42 days; c, 56 days; e, 56 days, g, 53 days; see Table 1 for details)