Table 1 The eight microsatellite loci used to examine genetic diversity in a sand fly Lutzomyia vexator at a site in northern California, the University of California Hopland Research and Extension Center. For each locus, the GenBank accession numbers, motif, length (in nucleotides) of expected amplicon at given repeat length and PCR primers are presented
Locus | GenBank | Motif | Length | Primers (5′–3′) |
|---|---|---|---|---|
Lvx9 | KT693036 | AG | 172 (19×) | F: CCGAATTGTCGAACGATTTG |
R: GTCAATTTGATGCTCTCGTAC | ||||
Lvx67 | KT693037 | AG | 165 (18×) | F: CCAAGAATCGCATATCAACATG |
R: GCTTCATCCTGTATTCATGG | ||||
Lvx123 | KT693038 | TC | 318 (22×) | F: CCTTATTCTCACTTGCATCTCGG |
R: AGAGAAGATAGAGCTCCATTGGG | ||||
Lvx179 | KT693039 | CT | 296 (22×) | F: CGCAAACATGCTGATAAAGAATGC |
R: GGACTTTGTTGCATTGCAGC | ||||
Lvx7442 | KT693040 | AG | 323 (19×) | F: GCTTCCAAAGAGGAAGGTGAG |
R: GGATACACTCGAAAATTGGTGC | ||||
Lvx504 | KT693041 | TC | 403 (25×) | F: GTTCTTTAAGACGCGTGAAATGC |
R: AAATTCTCATTGGGCAGGATAGC | ||||
Lvx90606 | KT693042 | CTT | 246 (12×) | F: GTCTCAATACGCGTCTCTCAAAG |
R: GTTAGTGCGAGAGGCAGAGTC | ||||
Lvx918 | KT693043 | AG | 301 (15×) | F: GGTTGAACAACAGTCAGCTAAG |
R: GCCTAAAGGAAACGTAGCATTCTC |