Organism | Primer name | Primer sequence (5'–3') | Gene (size, bp) | Amplification conditions | Reference |
---|---|---|---|---|---|
Legionella spp. (Identification) | LEG 225 | AAGATTAGCCTGCGTCCGAT | 16S rRNA (634) | 95 °C (1.5 min) followed by 30 cycles of 94 °C (10 s), 64 °C (1 min) and 74 °C (1 min). Final extension: 72 °C (10 min). | Miyamoto et al. [57] |
LEG 858 | GTCAACTTATCGCGTTTGCT | ||||
Legionella spp. | Leg F | CTAATTGGCTGATTGTCTTGAC | 23S-5S rRNA (259) | 95 °C (1 min) followed by 45 cycles of 95 °C (15 s), 60 °C (15 s) and 72 °C (11 s) | Herpers et al. [58] |
Leg R | CAATCGGAGTTCTTCGTG | ||||
Acanthamoeba spp. | AcantF900 | CCCAGATCGTTTACCGTGAA | 18S rDNA (±180) | 95 °C (1 min) followed by 45 cycles of 95 °C (15 s), 60 °C (1 min) and 72 °C (40 s) | Qvarnstrom et al. [59] |
AcantR1100 | TAAATATTAATGCCCCCAACTATCC | ||||
Naegleria fowleri | NaeglF192 | GTGCTGAAACCTAGCTATTGTAACTCAGT | 18S rDNA (153) | 95 °C (1 min) followed by 45 cycles of 95 °C (15 s), 64 °C (1 min) and 72 °C (1 min) | Qvarnstrom et al. [59] |
NaeglR344 | CACTAGAAAAAGCAAACCTGAAAGG | ||||
Vermamoeba (Hartmannella) vermiformis | Hv1227F | TTACGAGGTCAGGACACTGT | 18S rRNA (502) | 95 °C (3 min) followed by 45 cycles of 95 °C (20 s), 58 °C (30 s) and 72 °C (40 s) | Kuiper et al. [60] |
Hv1728R | GACCATCCGGAGTTCTCG |