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Fig. 3 | Parasites & Vectors

Fig. 3

From: Characterisation of tropomyosin and paramyosin as vaccine candidate molecules for the poultry red mite, Dermanyssus gallinae

Fig. 3

Immunoblots of native and recombinant Der g 10 and Der g 11 from Dermanyssus gallinae. a Tropomyosin. b Paramyosin. For both immunoblots: Lanes 1 and 6: Water soluble proteins; Lanes 2 and 7: Membrane associated proteins; Lanes 3 and 8: Integral membrane proteins; Lanes 4 and 9: Insoluble proteins; Lanes 5 and 10: Recombinant protein; Lane 11: Recombinant protein. Lanes 1–5 were probed with IgY extracted from egg yolk from hens immunised with either recombinant Der g 10 (a) or Der g 11 (b), and Lanes 6–10 were probed with control sera. Lane 11 was stained with SimplyBlue safe stain (Invitrogen). Lane M: SeeBlue plus 2 molecular mass markers, in kDa (Invitrogen). Stars indicate heavy and light chains of IgY in Lanes 1 and 2 for a, and 1, 2, 6 and 7 for b

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