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Table 2 Analytical sensitivity of the duplex PCR assay

From: Anaplasma marginale and A. phagocytophilum in cattle in Tunisia

Plasmid copiesa DNA uninfected cattleb A. phagocytophilum A. marginale
10 AP N Positive Negative
10 AP Y Positive Negative
1 AP N Negative Negative
1 AP Y Negative Negative
10 AM N Negative Positive
1 AM N Negative Positive
1 AM Y Negative Positive
103 AP + 10 AM N Positive Positive
103 AP + 10 AM Y Positive Positive
10 AP + 103 AM N Positive Positive
10 AP + 103 AM Y Positive Positive
  1. aAP, plasmid with an insert of the msp2 gene fragment of Anaplasma phagocytophilum; AM, plasmid with an insert of the msp4 gene fragment of Anaplasma marginale
  2. bPresence (Y) or absence (N) in the PCR reaction of DNA extracted from blood from a non-infected cow spiked with the indicated plasmid or plasmid combinations