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Table 2 Analytical sensitivity of the duplex PCR assay

From: Anaplasma marginale and A. phagocytophilum in cattle in Tunisia

Plasmid copiesa

DNA uninfected cattleb

A. phagocytophilum

A. marginale

10 AP

N

Positive

Negative

10 AP

Y

Positive

Negative

1 AP

N

Negative

Negative

1 AP

Y

Negative

Negative

10 AM

N

Negative

Positive

1 AM

N

Negative

Positive

1 AM

Y

Negative

Positive

103 AP + 10 AM

N

Positive

Positive

103 AP + 10 AM

Y

Positive

Positive

10 AP + 103 AM

N

Positive

Positive

10 AP + 103 AM

Y

Positive

Positive

  1. aAP, plasmid with an insert of the msp2 gene fragment of Anaplasma phagocytophilum; AM, plasmid with an insert of the msp4 gene fragment of Anaplasma marginale
  2. bPresence (Y) or absence (N) in the PCR reaction of DNA extracted from blood from a non-infected cow spiked with the indicated plasmid or plasmid combinations