Identification of internalin-A-like virulent proteins in Leishmania donovani

Parasites & Vectors

Table 2 Forward search analysis (hmmscan)

Query^a ID (Gene symbol)	Alternate protein IDs [Query]^a (UniProt, Ensembl, RefSeq GI)	Subject^b coverage (%)	Query^a length	Domain score^c	c-Evalue^d	i-Evalue^e	Query^a (Aligned region)	Sequence identity (%)	Conservation^f (%)
LdBPK_365070.1	E9BUL5, emb\|CBZ38944\|, gi\|398024920	81.49	1208	117	3.30e^-37	6.10e^-34	394–776	28.18	75.26
LdBPK_191670.1	E9BEF7, emb\|CBZ33633, gi\|398014298	55.74	560	141.6	1.10e^-44	2.00e^-41	265–526	26.98	77.78
LdBPK_030010.1	E9B7L9, emb\|CBZ31242\|, gi\|398009536	56.6	1009	117.4	3.10e^-37	4.50e^-34	202–467	23.05	75.78
LdBPK_030010.1	E9B7L9 emb\|CBZ31242\|, gi\|398009536	60.64	1009	145	1.30e^-45	1.90e^-42	563–847	28.52	78.15
LdBPK_051200.1	E9B8L0, emb\|CBZ31583, gi\|398010218	59.57	396	146	5.70e^-46	9.40e^-43	77–356	29.06	76.23
LdBPK_311630.1	E9BMT7, emb\|CBZ36565, gi\|398020181	72.13	811	213.8	1.60e^-66	2.70e^-63	394–732	30.65	84.21

Predicted distant Inl-A orthologs in L. donovani proteome identified based on hmmscan [14, 26] against a modified Pfam database [27, 28] (including an Inl-A profile)
^aQuery: corresponds to L. donovani proteins
^bSubject: corresponds to Internalin-A internalin domain profile
^cDomain Score: the calculated domain score for the best aligned profile (Inl-A)-sequence (L. donovani) matched states of the Inl-A-like internalin domain predicted in L. donovani proteins
^dc-Evalue (conditional E-value): measures the statistical significance of each domain, given that the target sequence is a true homolog. It is the expected number of additional domains one would find with a domain score this high in the set of sequences reported in the top hits list, if those sequences consisted only of random non-homologous sequence outside the region that sufficed to define them as homologs
^ei-Evalue (independent E-value): the significance of the sequence in the whole database search, if this were the only domain one had identified
^fConservation: estimation of identical or similar matching states over the alignment length

ISSN: 1756-3305