Fig. 3
From: Release of extracellular vesicles containing small RNAs from the eggs of Schistosoma japonicum
Schistosoma japonicum egg extracellular vesicles (EVs) and RNAs were internalized by murine liver cells. a Uptake analysis of S. japonicum egg EVs by mouse liver cells detected by confocal microscopy. PKH67-labeled S. japonicum egg EVs, Hepa1-6 EVs, and PHK67-PBS were incubated with Hepa1-6 cells for 1 h. Nuclei were stained with DAPI (blue). Scale-bars: 20 μm. b Relative level of parasite-derived miRNAs (i.e. bantam and miR-71b) in murine liver cells 20 h post-incubation with 10 μg S. japonicum egg EVs following PBS washing. The data were normalized to the level of miRNAs in 10 μg of EVs. *P ≤ 0.05