Fig. 3

Proliferation and cytokines secretion of T. spiralis-sensitized CD4⁺ T cells co-incubated with rTsPmy-pulsed DCs. To assess whether rTsPmy-pulsed DCs enable to stimulate T. spiralis-sensitized CD4⁺ T cells, the CD4⁺ T cells from splenocytes of T. spiralis infected BALB/c mice were incubated with rTsPmy-, LPS- or PBS-treated DCs and the proliferation of co-incubated T cells were determined by CFSE labeling and FACS (a, b). The levels of IFN-γ, IL-4, IL-10, TGF-β and IL-17A in the culture supernatants were measured by ELISA (c). Data are presented as the mean ± standard deviation from three individual experiments. *P < 0.05; **P < 0.01; ***P < 0.001