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Fig. 4 | Parasites & Vectors

Fig. 4

From: Trichinella spiralis paramyosin activates mouse bone marrow-derived dendritic cells and induces regulatory T cells

Fig. 4

Induction of CD4+CD25+ Foxp3+ T cell population and Treg cytokines in naïve T cells when incubated with rTsPmy-pulsed DCs. The splenocytes isolated from naïve BALB/c mice were incubated with rTsPmy-, LPS- or PBS-treated DCs for 36 h, then labeled with anti-CD4-FITC and anti-CD25-APC and anti-Foxp3-PE for FACS plot analysis (a). The percentage of CD4+CD25+Foxp3+ cells in CD4+ T cell population was showed in b. c Cytokine profile of naïve CD4+ T cells incubated with rTsPmy-, LPS- or PBS-treated DCs. The co-incubation supernatants were recovered and measured for secretions of IFN-γ, IL-4, IL-10, TGF-β and IL-17A by ELISA. Data are presented as the mean ± standard deviation from three individual experiments. *P < 0.05; **P < 0.01; ***P < 0.001 compared to PBS control

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