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Table 1 Primers used during this study

From: Novel Rickettsia raoultii strain isolated and propagated from Austrian Dermacentor reticulatus ticks

Name

Sequence (5′-3′)

Annealing temperature (°C)

Target

Reference

Rick-F1

GAACGCTATCGGTATGCTTAACACA

67–57a

16S ribosomal RNA gene

[23]

Rick-R2

CATCACTCACTCGGTATTGCTGGA

RCK/23-5-F

GATAGGTCRGRTGTGGAAGCAC

65–55a

23S–5S ribosomal RNA intergenic spacer

[29]

RCK/23-5-R

TCGGGAYGGGATCGTGTGTTTC

D1f

ATGAGTAAAGACGGTAACCT

60

sca4

[37]

D928r

AAGCTATTGCGTCATCTCCG

190-70

ATGGCGAATATTTCTCCAAAA

60

ompA

[38]

190-701

GTTCCGTTAATGGCAGCATCT

120-2788

AAACAATAATCAAGGTACTGT

60

ompB

[39]

120-3599

TACTTCCGGTTACAGCAAAGT

CS-78

GCAAGTATCGGTGAGGATGTAAT

60

gltA

[40]

CS-323

GCTTCCTTAAAATTCAATAAATCAGGAT

  1. a Touch down PCR protocol decreasing annealing temperature by 1 °C per cycle during the first 10 cycles