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Fig. 1 | Parasites & Vectors

Fig. 1

From: Identification of interchangeable cross-species function of elongation factor-1 alpha promoters in Babesia bigemina and Babesia bovis

Fig. 1

Definitive structure of the B. bigemina ef-1α locus. The structure of the B. bigemina ef-1α locus (upper panel) was deduced from the amplification of the PCR products obtained after amplification (lower panel). The previously 767 bp unknown sequence of the ef-1α locus is represented with a grey box. The location of primers used for the PCR amplification is marked with arrows. The regions on the IG containing IR repeats are represented by blue boxes, and the location of a ClaI site present in the IG region is also indicated with an arrow. PCR analysis performed on total B. bigemina gDNA amplicons on an agarose gel electrophoresis (lower panel). Lane 1, PCR product for Ig-A-Fwd + IG-FS-Rev primer; Lane 2, PCR product for Ig-B-Fwd + IG-FS-Rev. Molecular size marker is indicated on the left. Abbreviation: M, molecular size ladder in base pair (bp), 1000 bp DNA ladder

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