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Fig. 1 | Parasites & Vectors

Fig. 1

From: A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cells

Fig. 1

Expression of B. bovis merozoite surface antigens as soluble recombinant proteins in P. pastoris. a Schematic representation of MSA-2a1-His6, MSA-2b-His6 and MSA-2c-His6. Codon-optimized genes were synthesized and contained nucleotides corresponding to the indicated primary protein structures. Amino acids indicated in red represent modifications that were made to avoid N-glycosylation. b Genes were expressed as fusion recombinant proteins containing a hexa-histidine tag (His6) in Pichia pastoris yeast. The supernatants of yeast clones expressing each recombinant merozoite antigen were separated by SDS-PAGE under reducing conditions, stained with Coomassie Blue (left panel) and immunoblotted on nitrocellulose membranes using antibodies against the His6 tag (right panel). Lanes 1 and 4: MSA-2a1-His6; Lanes 2 and 5: MSA-2b-His6; Lanes 3 and 6: MSA-2c-His6

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