Specific antibody responses in mice immunized with recombinant B. bovis merozoite antigens. Immunization of C57BL/6 mice and measurement of antibody titers by ELISA was carried out according to the timeline described in a. Mice were divided in five groups (n = 5 in each group) and immunized with three doses of the following: (1) Montanide ISA 720; (2) Montanide ISA 720 plus rMSA-2a1 (10 μg/dose/mouse); (3) Montanide ISA 720 plus rMSA-2b (10 μg/dose/mouse); (4) Montanide ISA 720 plus rMSA-2c (10 μg/dose/mouse); (5) Montanide ISA 720 plus a protein mix (rMSA-2a1, rMSA-2b, rMSA-2c; 10 μg of each protein per mouse). Serum IgG antibody titers against B. bovis merozoite antigens were determined by ELISA using each recombinant protein in the plate. b rMSA-2a1.
c rMSA-2b. d rMSA-2c. The results represent the mean values ± standard error obtained for each group