Fig. 5From: A recombinant multi-antigen vaccine formulation containing Babesia bovis merozoite surface antigens MSA-2a1, MSA-2b and MSA-2c elicits invasion-inhibitory antibodies and IFN-γ producing cellsIndirect immunofluorescence assay for the recognition of native MSA-2a1, MSA-2b and MSA-2c. Smears of B. bovis-infected erythrocytes (CC) were incubated with pools of sera from mice immunized with each recombinant protein (1:100 dilutions) or control sera (1:50 dilution). Reactions were detected with FITC-conjugated anti-mouse IgG antibodies (FITC). Nuclei were detected by DAPI staining (DAPI). Slides were observed with 400× magnification. Scale-bars: 10 μmBack to article page