Fig. 7
Cell-mediated immunity of mice immunized with recombinant MSA antigens. C57BL/6 mice were immunized with three doses, 21 days apart, of a mixture containing either 1 μg or 10 μg of each protein/dose (per mouse) emulsified in Montanide ISA 720. Control mice received adjuvant only. Splenic cells of 4 mice were cultured in the presence of anti-CD28, monensin and brefeldin-A with or without the recombinant proteins or ConA, as indicated. After 12 h, cells were stained with anti-CD4, anti-CD8, anti-IFN-γ, and anti-TNFα. a Total frequencies (%) of CD4+ or CD8+ cells stained for TNF-α and IFN-γ b and c Mean frequencies of 4 mice ± SE of IFN-γ+ CD4 or CD8 cells, respectively. The results are representative of two experiments. Asterisks denote statistically significant (P = 0.009 for rMSA-2a1 and P = 0.045 for rMSA-2c) higher frequencies of cells from mice immunized with the mixture of proteins when compared to cells from mice injected with adjuvant only