Fig. 4

Localization of EtSIR2A in infected DF-1 cells by indirect immunofluorescence. Details of parasites immunostained with anti-rEtSIR2A antibodies visualized with fluorescein isothiocyanate (green) and counterstained with DAPI (blue). a Sporozoites (Spz) incubated in PBS. Infected DF-1 cells were collected at different times post-infection (p.i.). b 12 h p.i., intracellular sporozoites (iSpz); c 24 h p.i., intracellular sporozoites (iSpz); d 36 h p.i., trophozoites (Tropho); e 48 h p.i., immature first-generation schizonts (iSc); f 68 h p.i., developing first-generation schizonts (dSc); g 72 h p.i., mature first-generation schizonts (mSc); h 112 h p.i., second-generation merozoites (sMz) purified from caeca. Abbreviations: N: nucleus; pRB, posterior refractile body; fMz, first generation merozoites