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Table 1 Comparison of TSSApep ELISA reactions with haemoculture and genotyping data. All samples had tested positive for T. cruzi infection with IFAT. Seven samples reacted non-specifically and are excluded (see text)

From: Lineage-specific serology confirms Brazilian Atlantic forest lion tamarins, Leontopithecus chrysomelas and Leontopithecus rosalia, as reservoir hosts of Trypanosoma cruzi II (TcII)

Species

Biome

Region

Haemoculture

Genotypinga

nb

TSSA peptide reaction

 
      

II/V/VI

III

IV

V/VI

Lysatec

L. chrysomelas

Atlantic Rainforest

Una, Bahia

Positive

I

1

1

0

0

1/1 of TSSA II/V/VI

0

    

II

11

8

0

0

6/8 of TSSA II/V/VI

5 (1)

    

Uncharacterised

6

4

0

0

4/4 of TSSA II/V/VI

3 (1)

   

Negative

 

8

2

0

0

1/2 of TSSA II/V/VI

5 (4)

L. chrysopygus

Atlantic Rainforest

Guapimirim, RJ

Positive

II

1

0

0

0

0

0

L. rosalia

Atlantic Rainforest

Silva Jardim, RJ

Positive

II

8 (4)

3

0

0

1/3 of TSSA II/V/VI

2

    

Mixed I/II

1 (1)

1

0

0

1/1 of TSSA II/V/VI

1

    

Uncharacterised

1

1

0

0

0

1

   

Negative

 

3

3

0

0

0

3

S. libidinosus

Amazon

Estreito, Maranhão

Positive

Uncharacterised

2

0

1d

1d

0

1d

   

Negative

 

4

0

0

0

0

0

A. bezebul

Amazon

Estreito, Maranhão

Positive

Mixed I/IV

1

0

0

0

0

0

    

Uncharacterised

1

0

0

0

0

1 (1)

   

Negative

 

5

0

0

0

0

0

  1. aAll samples genotyped by PCR of the mini-exon
  2. bParentheses indicate TcII infections confirmed by PCR:RFLP based on SNPs in the HSP60 and GPI loci and by PCR of LSU rDNA
  3. cParentheses indicate samples that reacted with lysate only
  4. dSame sample reacting with TSSApep-III and TSSApep-IV and lysate