Fig. 6

Effect of triatomine saliva on cytokine production and T. cruzi infection in DCs. Murine bone marrow-derived DCs were incubated with saliva (1:100 v/v) and CFSE-labeled T. cruzi (Y strain) (1 nM CFSE; at a parasite-cell ratio of 3:1) and fluorescent DCs were quantified by flow cytometry after 18 h of culture (a-g). a Percent of T. cruzi-infected DCs. h-j The culture supernatant was collected and analyzed to detect IL-6 (h), IL-12 p40 (i), and IL-10 (j) by ELISA. Bars represent the mean ± standard error of the invasion and cytokine production in cultured DCs. *P < 0.05 compared with DCs cultured with T. cruzi only. Abbreviations: P.l, P. lignarius; M.p, M. pallidipennis; T.l, T. lecticularia; R.p, R. prolixus