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Table 1 Sampling sites, collection data and HRMA blood meal analysis results according to DNA extraction method

From: Identification of Ixodes ricinus blood meals using an automated protocol with high resolution melting analysis (HRMA) reveals the importance of domestic dogs as larval tick hosts in Italian alpine forests

DNA extraction method

Sampling sitea

Nearest locality to sampling site

Sampling time

%

identification successb

No. of

mixed blood meals

Mean identification success (%)

QiaAMP® DNA Investigator

BREc

Brentonico

May 2012

46.7 (7/15)

1

55.1

CADc

Cadine

May 2012

35.7 (5/14)

0

CON

Condino

April 2012

69.2 (9/13)

4

GIOc

Giovo

April 2013

43.8 (7/16)

1

GRIb

Grigno Valsugana

April 2013

31.3 (5/16)

0

PIE

Pietramurata

May 2012

92.3 (12/13)

2

TRA

Transacqua

June 2012

66.7 (8/12)

2

ThermoScientific KingFisherTM

ALA

Ala

May 2012

33.3 (10/30)

2

22.4

BREc

Brentonico

May 2012

29.4 (5/17)

0

CAO

Caoria

April 2013

30.0 (9/30)

2

CADc

Cadine

May 2012

25.0 (2/8)

0

CAV

Cavedine

April 2013

20.0 (6/30)

0

CVS

Cavalese

May 2012

21.7 (5/23)

0

DIM

Dimaro

May 2013

33.3 (10/30)

2

GIOc

Giovo

April 2013

31.3 (5/16)

0

GRIc

Grigno Valsugana

April 2013

43.8 (7/16)

0

LAM

Laghi di Lamar

May 2012

9.7 (3/31)

0

LED

Ledro

May 2012

20.0 (6/30)

0

LEV

Levico

April 2012

26.7 (8/30)

0

LUN

Lundo

May 2012

6.7 (2/30)

0

MEZ

Mezzocorona

June 2012

16.7 (5/30)

2

MOL

Molveno

May 2012

3.3 (1/30)

0

PDU

Passo del Durone

June 2012

32.4 (11/34)

0

PER

Pergine

April 2012

20.0 (6/30)

1

PIN

Pinzolo

May 2012

20.0 (6/30)

0

REV

Revò

May 2012

13.3 (4/30)

0

SEG

Segonzano

May 2012

23.3 (7/30)

1

 

TEL

Telve

June 2012

13.3 (4/30)

1

 

TES

Tesino

June 2012

20.0 (6/30)

0

 

TIO

Tione di Trento

May 2012

10.0 (3/30)

0

 

TRE

Trento

May 2013

17.6 (6/34)

0

 

VER

Vervò

May 2012

20.0 (6/30)

0

 

VGE

Val Genova

May 2013

13.3 (4/30)

0

 

VOL

Volano

May 2012

50.0 (15/30)

2

 
  1. a Abbreviations as in Fig. 1
  2. bNumber of nymphs with identified blood meal/number of nymphs analysed (as shown in parentheses)
  3. cSite with samples extracted with either QiaAMP® DNA Investigator or ThermoScientific KingFisherTM methods