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Fig. 4 | Parasites & Vectors

Fig. 4

From: A mitochondrial HSP70 (HSPA9B) is linked to miltefosine resistance and stress response in Leishmania donovani

Fig. 4

Miltefosine incorporation in transgenic L. donovani parasites. Cells were incubated with 5 μM MIL-BODIPY and subsequently washed with 4% BSA in HPMI buffer (w/v) and analyzed by flow cytometry. a MIL-BODIPY uptake in GFP-HSPA9B-pXG (green filled line) and mock parasites (black line). b MIL-BODIPY accumulation in HSPA9B-mCherry (red filled line) and WT parasites (black line). Culture auto-fluorescence is indicated as dotted lines. These are representative histograms of at least three independent experiments

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