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Fig. 5 | Parasites & Vectors

Fig. 5

From: A mitochondrial HSP70 (HSPA9B) is linked to miltefosine resistance and stress response in Leishmania donovani

Fig. 5

Subcellular localization of HSPA9B. a Immunofluorescence microscopy of HSPA9B-mCherry promastigotes (red) using a primary polyclonal antibody against lipoic acid and a FITC-conjugated anti-rabbit (green) as a secondary antibody. DNA was stained with NucBlue fixed cell stain (DAPI, blue). Scale-bar: 5 μm. Images were obtained with a Nikon 90/NikonAZ 100 microscope. b Western blot of subcellular fractions obtained by digitonin fractioning of HSPA9B-mCherry parasites. Samples were separated by SDS-PAGE, transferred on to a PVDF membrane and assayed with anti-mCherry antibody. The membrane was stripped and re-probed with a polyclonal anti-lipoamide antibody. Molecular weights are indicated in kDa. Fractions (F) 1 and 2 contain cytosolic proteins, mitochondrial proteins are mostly recovered in fractions (F) 3 and 4; fraction (F) 5 is enriched in cytoskeleton and cytoskeleton-associated proteins

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