Fig. 6

Environmentally-induced stress response and ROS generation in L. donovani overexpressing HSPA9B. a Promastigotes were exposed to low pH (pH = 5.5), heat-shock (37 °C), heat-shock (37 °C) plus low pH (pH = 5.5), starvation and HePC treatment. Control cells were incubated at 27 °C. The percentage (%) of early apoptotic-AnnexinV positive cells and late apoptotic-PI positive cells was measured by flow cytometry. These are representative histogram bars of at least two independent experiments. b Production of intracellular ROS under low pH (pH = 5.5), heat-shock (37 °C) plus low pH (pH = 5.5) and HePC treatment was measured by flow cytometry using MitoSOX Red. Data are the means ± standard deviations (SD) of three independent experiments. *P < 0.05