Fig. 8

Effect of HSPA9B overexpression on differentiation. a L. donovani WT and transgenic promastigotes (HSPA9B-mCherry) were inoculated in low pH (pH = 5.5) medium and 37 °C to trigger differentiation to axenic amastigotes. Cells were collected 24 and 48 h post-induction of differentiation and either lysed for western blotting with axenic amastigote A2 marker and anti-tubulin as a loading control or counted using a Neubauer chamber. b The molecular weight of standard proteins is indicated in kDa and the mean ± standard deviation (SD) of triplicate determinations are shown, respectively