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Fig. 1 | Parasites & Vectors

Fig. 1

From: An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies

Fig. 1

Design of a LAMP assay targeting O. volvulus cox1. a Alignment of partial gene sequences of cytochrome c oxidase subunit 1 from O. volvulus, W. bancrofti, L. loa, B. malayi and O. ochengi. b Primer set targeting O. volvulus cox1. c Species-specific LAMP assay targeting O. volvulus cox1. c Dilution series of gBlocks containing the cox1 fragment of O. volvulus, W. bancrofti, L. loa and B. malayi were used as template in the LAMP assay using real-time fluorescent dye detection. All samples were analyzed in duplicate. Dilution indicated with an asterisk had detectable signal in only one of both duplicates

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