Table 3 Classification of phylogeographic studies based on review categories

Spatial scale

Continental

5

[67, 68, 140–142]

National

4

[77, 78, 143, 144]

Regional

5

[64, 80, 81, 84, 145]

Aims

Phylogenetic relationships

4

[64, 142, 144, 145]

Genetic diversity and phylogenetic relationships

2

[78, 141]

Genetic diversity and spatial associations

4

[67, 68, 81, 143]

Population structure and spatial associations

2

[77, 84]

Spatial association and haplotype-host association

2

[80, 140]

Hypothesis

Study and sample design based on a hypothesis

2

[80, 84]

Study and sample design not based on a hypothesis

12

[64, 67, 68, 77, 78, 81, 140–145]

Sample source

Humans, other mammals and triatomines

8

[64, 68, 80, 84, 140–142, 144]

Triatomines and other mammals

5

[67, 77, 78, 81, 145]

Triatomines

1

[143]

Parasite population analyzed

Isolates selected using in vitro or in vivo methods

11

[64, 67, 68, 77, 78, 81, 140–142, 144, 145]

Original sample

3

[80, 84, 143]

Molecular marker used for diagnosis and genotyping

Mini-exon

3

[80, 140, 144]

24S rRNA

1

[67]

24S rRNA + mini-exon

3

[64, 68, 142]

S35/S36 kDNA

2

[81, 143]

S35/S36 + TcZ1/TcZ2 + mini-exon + 24S rRNA + 18S rRNA

1

[145]

MLEE +/or RAPD+ miniexon

1

[84]

LSU rDNA + HSP60 + GPI

1

[77]

GPI

2

[78, 141]

DTU classification

Classified in study

13

[64, 67, 68, 77, 78, 80, 81, 84, 141–145]

Previously classified

1

[140]

Population genetic analysis

Nuclear sequences

3

[80, 140, 145]

Nuclear + mitochondrial sequences

2

[64, 142]

Microsatellites

3

[68, 81, 143]

Nuclear sequence and microsatellite

1

[67]

Mitochondrial sequence and microsatellite

3

[77, 78, 141]

RFLP or LSSP-PCR

2

[84, 144]

Outgroups

T. c. marinkelli

1

[67]

T. c. marinkelli + T. dionisii

1

[142]

DTUI

1

[77]

DTUII

2

[84, 144]

DTUIII + DTUIV

1

[141]

DTUIV

1

[78]

none

7

[64, 68, 80, 81, 140, 143, 145]