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Fig. 2 | Parasites & Vectors

Fig. 2

From: Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

Fig. 2

Photographs of agarose electrophoresis gels (2%) for PCR-profiling with a panel of Cry, Cyt and Chi gene primers. From left to right and for all panels: Lane 1: 100 bp ladder; Lane 2: reference Bti-H14; Lanes 3–25: the 23 native Bt strains indicated by their corresponding identification numbers (see Table 3). In a, b, df, all 23 native Bt strains including Bti-H14 displayed positive amplification of Cyt1, Cyt2, Cry4B, Cry10, Cry11, Cyt1Aa and Cyt2Aa. In c, all strains were positive for Cry4A except Bt63. In g, all Bt strains were PCR negative for Chi gene except Bt-12 and 55; whereas all Bt strains were PCR positive for Cyt1Ab gene, except the native isolates coded 67, 60, 63, 56 and 16

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