Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

Table 4 PCR-amplification profiles for the cry, cyt and chi genes of eight native Bt strains significantly more bioactive that the Bti-H14 reference strain against An. gambiae larvae

Strain/Accession No.^a	Mean LC₅₀ (μg/ml) (range)^b	Positive cry, cyt, and chi genes
		cry4Aa	cry4Ba	cry10	cry11	cyt1Aa	cyt1Ab	cyt2Aa	chi
Bti-H14 - KJ722438.1	13.33 (11.2–15.9)	+	+	+	+	+	+	+	no
Bt10 - KF150482.1	4.96 (4.41–5.58)^*	+	+	+	+	+	+	+	no
Bt42 - KC414685.1	5.90 (5.2–6.8)^*	+	+	+	+	+	+	+	no
Bt55 - KC527056.1	4.11 (3.6–4.6)^*	+	+	+	+	+	+	+	+
Bt57 - KC527056.1	7.40 (6.5–8.25)^*	+	+	+	+	+	+	+	no
Bt58	5.73 (5.06–6.5)^*	+	+	+	+	+	+	+	no
Bt63 - KF971833.1	3.90 (3.4–4.4)^*	no	+	+	+	+	no	+	no
Bt67 - KC527056.1	6.90 (5.9–8.2)^*	+	+	+	+	+	no	+	no
Bt68 - KC527056.1	5.16 (4.5–5.8)^*	+	+	+	+	+	+	+	no

Abbreviation: no indicates absence of PCR-amplification
^*Asterisks indicate significantly greater bioactivity compared to BtiH14 (see Table 3)
^a16s RNA sequences accession number when applicable
^bMean and range of lethal concentrations (LC₅₀)

ISSN: 1756-3305