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Table 4 PCR-amplification profiles for the cry, cyt and chi genes of eight native Bt strains significantly more bioactive that the Bti-H14 reference strain against An. gambiae larvae

From: Isolation and characterization of native Bacillus thuringiensis strains from Saudi Arabia with enhanced larvicidal toxicity against the mosquito vector Anopheles gambiae (s.l.)

Strain/Accession No.a Mean LC50 (μg/ml) (range)b Positive cry, cyt, and chi genes
   cry4Aa cry4Ba cry10 cry11 cyt1Aa cyt1Ab cyt2Aa chi
Bti-H14 - KJ722438.1 13.33 (11.2–15.9) + + + + + + + no
Bt10 - KF150482.1 4.96 (4.41–5.58)* + + + + + + + no
Bt42 - KC414685.1 5.90 (5.2–6.8)* + + + + + + + no
Bt55 - KC527056.1 4.11 (3.6–4.6)* + + + + + + + +
Bt57 - KC527056.1 7.40 (6.5–8.25)* + + + + + + + no
Bt58 5.73 (5.06–6.5)* + + + + + + + no
Bt63 - KF971833.1 3.90 (3.4–4.4)* no + + + + no + no
Bt67 - KC527056.1 6.90 (5.9–8.2)* + + + + + no + no
Bt68 - KC527056.1 5.16 (4.5–5.8)* + + + + + + + no
  1. Abbreviation: no indicates absence of PCR-amplification
  2. *Asterisks indicate significantly greater bioactivity compared to BtiH14 (see Table 3)
  3. a16s RNA sequences accession number when applicable
  4. bMean and range of lethal concentrations (LC50)