Fig. 2

Expression and identification of rCsCP and CotC-CsCP. a SDS-PAGE analysis of CsCP expressed in E. coli BL21 and B. subtilis spores. The molecular mass of CotC-CsCP fusion protein was approximately 43.8 kDa. Abbreviations: B.s-CotC-CP, WB600 containing pEB03-CotC-CsCP; B.s-CotC, WB600 harbouring pEB03-CotC; BL21-CP, BL21 harbouring pET28a-CsCP; rCP, purified rCsCP. b The expression of CotC-CsCP fusion protein at different sporulation times by 12% SDS-PAGE. c Total spore coat proteins extracted from recombinant spores (pEB03-CotC-CsCP) by SDS-PAGE analysis. d Identification of CotC-CsCP fusion protein by MS. e MALDI-TOF/TOF-MS analysis of purified rCsCP. f Expression identification of CotC-CsCP fusion protein at different sporulation times by Western blotting using rat anti-rCsCP serum. g Total coat proteins of pEB03-CotC-CsCP spore recognized by rat anti-rCsCP serum using Western blotting. Abbreviations: P, precipitation; S, supernatant