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Fig. 3 | Parasites & Vectors

Fig. 3

From: The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 3

Expression identification of CsCP on the coat of recombinant spores by immunofluorescence. The B. subtilis spores with pEB03-CotC-CsCP were observed by immunofluorescence (a) and confocal laser microscope (b) after incubating with rat anti-CsCP serum and Cy3 labeled goat anti-rat IgG (red). The nucleus was stained with DAPI (blue). Sporulation CotC strain treated with the same method and both visualized under fluorescent light (c). All spores above were observed under bright field (BF) as well. Abbreviations: B.s-CotC-CP, WB600 containing pEB03-CotC-CsCP; B.s-CotC, WB600 harbouring pEB03-CotC. Scale-bars: a, c, 50 μm; b, 2 μm

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Parasites & Vectors

ISSN: 1756-3305

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