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Fig. 5 | Parasites & Vectors

Fig. 5

From: The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease

Fig. 5

ELISA assay of immune responses triggered by the oral administration of recombinant B. subtilis spores. Specific IgG (a), IgG1/IgG2a (b), and IgA (c) levels in sera from mice orally treated with pEB03-CotC-CsCP- or pEB03-CotC-transformed spores, BL21-CsCP and PBS were detected. CsCP-specific IgG (d) and sIgA (e) levels in intestinal mucous and sIgA level in bile (f) were analysed. Data are expressed as the mean ± SD. Statistical significance was analysed by the Student’s t-test (*P < 0.05; **P < 0.01). Abbreviations: B.s-CotC-CP, WB600 containing pEB03-CotC-CsCP; B.s-CotC, WB600 containing pEB03-CotC; BL21-CP, BL21 harbouring pET28a-CsCP

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Parasites & Vectors

ISSN: 1756-3305

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