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Fig. 2 | Parasites & Vectors

Fig. 2

From: Involvement of nucleoside diphosphate kinase b and elongation factor 2 in Leishmania braziliensis antimony resistance phenotype

Fig. 2

SbIII susceptibility assay of clonal lines from L. braziliensis (a) and L. infantum (b) non-transfected or transfected with the constructs pIR1BSD or pIR1BSD-NDKb. Parasites were incubated in M199 medium in the absence or presence of different concentrations of SbIII (1.17 to 599.04 μM) for 48 h and the percentage of relative growth was determined using a Z1 Coulter Counter. Mean values ± standard deviations from three independent experiments in triplicate are shown. Statistical analysis was carried out using Student’s t-test. Statistically different values are highlighted as follows: *P < 0.05; **P < 0.01; ***P < 0.001. Pairwise comparisons (a): 1.17 μM: LbWTS vs LbNDKb clone 4 (t (5) = 9.47, P = 0.0002); LbWTS vs LbNDKb clone 9 (t (9) = 3.36, P = 0.0084); 2.34 μM: LbWTS vs LbNDKb clone 4 (t (5) = 4.3, P = 0.0077); LbWTS vs LbNDKb clone 9 (t (8) = 3.74, P = 0.0057); 4.68 μM: LbWTS vs LbNDKb clone 4 (t (4) = 4.02, P = 0.0159); LbWTS vs LbNDKb clone 9 (t (5) = 4.99, P = 0.0042); 9.36 μM: LbWTS vs LbNDKb clone 4 (t (6) = 8.39, P = 0.0002); LbWTS vs LbNDKb clone 9 (t (8) = 12.50, P < 0.0001); 18.72 μM: LbWTS vs LbNDKb clone 4 (t (8) = 2.88, P = 0.0206); LbWTS vs LbNDKb clone 9 (t (10) = 7.36, P < 0.0001)

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