Fig. 3
SbIII susceptibility assay of clonal lines from L. braziliensis (a) and L. infantum (b) non-transfected or transfected with the constructs pIR1BSD or pIR1BSD-EF2. Parasites were incubated in M199 medium in the absence or presence of different concentrations of SbIII (1.17 to 599.04 μM) for 48 h and the percentage of relative growth was determined using a Z1 Coulter Counter. Mean values ± standard deviations from three independent experiments in triplicate are shown. Statistical analysis was carried out using Student’s t-test. Statistically different values are highlighted as follows: *P < 0.05; **P < 0.01; ***P < 0.001. Pairwise comparisons (a): 1.17 μM: LbWTS vs LbEF2 clone 9 (t(9) = 6.5, P = 0.0001); LbWTS vs LbEF2 clone 12 (t(5) = 2.84, P = 0.0361); 2.34 μM: LbWTS vs LbEF2 clone 9 (t(7) = 6.95, P = 0.0002); LbWTS vs LbEF2 clone 12 (t(4) = 2.87, P = 0.0455); 4.68 μM: LbWTS vs LbEF2 clone 9 (t(5) = 6.04, P = 0.0018); LbWTS vs LbEF2 clone 12 (t(4) = 2.87, P = 0.0453); 9.36 μM: LbWTS vs LbEF2 clone 9 (t(7) = 22.18, P < 0.0001); LbWTS vs LbEF2 clone 12 (t(8) = 4.48, P = 0.0021); 18.72 μM: LbWTS vs LbEF2 clone 9 (t(11) = 4.74, P = 0.0006); LbWTS vs. LbEF2 clone 12 (t(9) = 7.65, P < 0.0001); 37.44 μM: LbWTS vs LbEF2 clone 9 (t(5) = 2.78, P = 0.0391); LbWTS vs LbEF2 clone 12 (t(5) = 5.1, P = 0.0038). (b) 18.72 μM: LiWTS vs LiEF2 clone 5 (t(9) = 2.67, P = 0.0257); 37.44 μM: LiWTS vs LiEF2 clone 5 (t(8) = 6.14, P = 0.0003); 74.88 μM: LiWTS vs LiEF2 clone 5 (t(9) = 4.9, P = 0.0008)