Fig. 4
EC50 of lamivudine for wild-type and NDKb-overexpressing L. braziliensis lines (a) and effect of lamivudine on the growth of L. braziliensis lines upon SbIII exposure (b). Parasites were incubated in M199 medium in the absence or presence of different concentrations of lamivudine (C8H11N3O3S) (125 to 10,000 μM). For competition assay, cells were exposed to the EC50 of SbIII (7, 12 and 15 μM for the LbWTS and NDKb-overexpressing clones 4 and 9, respectively) and the EC50 of lamivudine (766, 2110 and 2014 μM for the LbWTS and NDKb-overexpressing clones 4 and 9, respectively) independently or combined, followed by incubation for 48 h. The percentage of relative growth was determined using a Z1 Coulter Counter. Mean values ± standard deviations from three independent experiments in triplicate are shown. Statistical analysis was carried out using Student’s t-test. Statistically different values are highlighted as follows: **P < 0.01; ***P < 0.001. Pairwise comparisons (a): 250 μM: LbWTS vs LbNDKb clone 9 (t(7) = 4.04, P = 0.0049); 500 μM: LbWTS vs LbNDKb clone 4 (t(9) = 5.27, P = 0.0005); LbWTS vs LbNDKb clone 9 (t(9) = 3.74, P = 0.0046); 1000 μM: LbWTS vs LbNDKb clone 4 (t(10) = 5.67, P = 0.0002); LbWTS vs LbNDKb clone 9 (t(10) = 3.52, P = 0.0055); 2500 μM: LbWTS vs LbNDKb clone 4 (t(8) = 5.03, P = 0.0010); LbWTS vs LbNDKb clone 9 (t(8) = 3.91, P = 0.0045)