Table 1 EC50 of SbIII, lamivudine, EF2K inhibitor and hydrogen peroxide and corresponding RI for WTS, NDKb- or EF2-overexpressing L. braziliensis lines. EC50 represents effective concentration required to decrease growth by 50%. The values were determined from at least three independent experiments performed in triplicate, using the linear interpolation method [38]
Parasites | SbIII | Lamivudine | EF2K inhibitor | Hydrogen peroxide | ||||
|---|---|---|---|---|---|---|---|---|
EC50 (μM) | RI | EC50 (μM) | RI | EC50 (μM) | RI | EC50 (μM) | RI | |
LbWTS | 7 | – | 766 | – | 173 | – | 213 | – |
LbpIR1BSD | 8 | – | 733 | – | – | – | 207 | – |
LbNDKb clone 4 | 12 | 1.7 | 2,110 | 2.8 | – | – | 207 | 1.0 |
LbNDKb clone 9 | 15 | 2.1 | 2,014 | 2.6 | – | – | 207 | 1.0 |
LbEF2 clone 9 | 14 | 2.0 | – | – | 211 | 1.2 | – | – |
LbEF2 clone 12 | 11 | 1.6 | – | – | – | – | – | – |