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Table 1 Evaluation of TB effect of anti-rPSOP25 serum and mAb in mosquito feeding assays

From: Functional characterization of Plasmodium berghei PSOP25 during ookinete development and as a malaria transmission-blocking vaccine candidate

 

Immunization groupsa

Monoclonal antibody transfer groupsb

Control mice

rPSOP25 immunized mice

Control mice

mAb transferred mice

M1

M2

M3

M1

M2

M3

M1

M2

M3

M1

M2

M3

No. of mosquitoes infected/dissected

30/30

30/30

29/30

20/28

20/30

19/28

28/29

27/28

28/28

22/30

21/29

20/29

Prevalence of infection (%)c

100

100

99.7

71.4

66.7

67.9

96.6

96.4

100

73.3

72.4

69.0

Mean prevalence (%)

99.9

68.7*

  

97.7

  

71.6*

Reduction in prevalence (%)d

     

31.2

     

26.1

Oocyst intensitye

90.7

86.8

90.5

25.2

22.1

21.7

97.1

96.3

94.3

29.8

34.2

33.7

SEMf

11.58

11.17

12.50

5.890

5.232

5.138

13.47

13.66

11.82

5.462

6.373

6.800

Mean oocyst intensity

  

89.3

  

23.0*

  

95.9

  

32.6*

Reduction in oocyst intensity (%)g

     

66.3

     

63.3

  1. aTB activity assay was carried out using rPSOP25-immunized mice
  2. bTB activity assay was carried out using BALB/C mice transferred with the anti-PSOP25 mAb
  3. cThe prevalence of infection was calculated by the number of mosquitoes with oocysts/total mosquitoes dissected in each group × 100%
  4. dThe percent reduction of prevalence was calculated as % mean prevalence control – % mean prevalence PSOP25
  5. eMean number of oocysts per mosquito midgut
  6. fStandard error of the mean
  7. gThe percent reduction in oocyst intensity was calculated as (mean oocyst intensity control – mean oocyst intensity PSOP25)/mean oocyst intensity control × 100%
  8. *P < 0.001 for comparisons between the experimental group and the control group