Table 1 Primers used in the study, annealing temperatures used in the PCRs and expected sizes of the PCR products
From: The presence of Giardia intestinalis in donkeys, Equus asinus, in China
Gene | Primer | Sequence (5'-3') | Annealing temperature (°C) | Fragment length (bp) | Reference |
|---|---|---|---|---|---|
tpi | F1 | AAATIATGCCTGCTCGTCG | 55 | 530 | [24] |
R1 | CAAACCTTITCCGCAAACC | ||||
F2 | CCCTTCATCGGIGGTAACTT | 55 | |||
R2 | GTGGCCACCACICCCGTGCC | ||||
gdh | F1 | TTCCGTRTYCAGTACAACTC | 50 | 530 | [24] |
R1 | ACCTCGTTCTGRGTGGCGCA | ||||
F2 | ATGACYGAGCTYCAGAGGCACGT | 65 | |||
R2 | GTGGCGCARGGCATGATGCA | ||||
bg | F1 | AAGCCCGACGACCTCACCCGCAGTGC | 50 | 511 | [24] |
R1 | GAGGCCGCCCTGGATCTTCGAGACGAC | ||||
F2 | GAACGAACGAGATCGAGGTCCG | 60 | |||
R2 | CTCGACGAGCTTCGTGTT |