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Fig. 2 | Parasites & Vectors

Fig. 2

From: Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system

Fig. 2

Expression, auto-activation and toxicity tests for the TgGRA15 bait. a Western blotting analysis of total protein extracts of Y2HGold containing the following plasmids: Lane 1: pGBKT7-TgGRA15; Lane 2: pGBKT7-53. b Determination of the auto-activation and toxicity activity of the pGBKT7-TgGRA15 bait plasmid in yeast cells. The pGBKT7-TgGRA15 bait plasmid and empty pGBKT7 vector were used to transform Y2HGold cells and then grown on different plates

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