Fig. 2From: Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid systemExpression, auto-activation and toxicity tests for the TgGRA15 bait. a Western blotting analysis of total protein extracts of Y2HGold containing the following plasmids: Lane 1: pGBKT7-TgGRA15; Lane 2: pGBKT7-53. b Determination of the auto-activation and toxicity activity of the pGBKT7-TgGRA15 bait plasmid in yeast cells. The pGBKT7-TgGRA15 bait plasmid and empty pGBKT7 vector were used to transform Y2HGold cells and then grown on different platesBack to article page