Fig. 8
Leukocytes characterization in tick-infested skins. a Paraffin-fixed sections were stained with May-Grünwald and Giemsa to differential counts of local granulocytes. The granulocytes were counted into the dermis of tick-resistant (white boxes) and tick-susceptible (gray boxes) from areas of 0.0625 mm2, and the means of each bovine were used for further analyses. Values followed by the same capital letter differ significantly (P < 0.05), intra-group comparison. Asterisks indicate significant differences in inter-breed comparisons: *P < 0.05; **P < 0.001; b Areas of inflammation in nymph-bitten skin from tick-resistant and tick-susceptible bovines presented conspicuous granules (blackarrows) densely dispersed in the lesion. Scale-bar: 1 μm