Immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick Rhipicephalus microplus

Table 4 Effect of the origin of blood meal on transcription of genes encoding secreted immunomodulatory and matrix modulatory proteins in the cattle tick Rhipicephalus microplus. Non-normalized cDNA libraries were constructed from salivary glands of nymphs fed on tick-susceptible (^a) or tick-resistant hosts (^b), from unfed larvae ecloded from eggs oviposited by females fed on tick-susceptible (^c) or tick-resistant hosts (^d), from unfed larvae obtained as in (^c) and exposed to skin volatiles of tick-susceptible hosts (^e) or tick-resistant hosts (^f) and from larvae obtained as in (^c) and fed for 24 on tick-susceptible (^g) and tick-resistant (^h) hosts

Functional class of predicted tick secreted protein	Library	Number of reads		Library	Number of reads
Functional class of predicted tick secreted protein	Library	Observed	Expected	Library	Observed	Expected	P-value χ ² test
Evasins
	^aSGNS	83	49	^bSGNR	6	40	< 0.001
DAP36 immunossupressant family
	SGNS	34	20	SGNR	2	16	< 0.001
SAPL15 immunossupressant family
	SGNS	40	29	SGNR	13	24	0.004
Chitinases
	^cULS	16	25	^dULR	22	13	0.004
	SGNS	6	13	SGNR	17	10	0.010
Cysteine proteases with possible basophil activation activity
	ULS	7	13	ULR	13	7	0.009
	^eULVS	221	341	^fULVR	149	20	< 0.001
	^gLS	73	92	^hLR	38	20	< 0.001
	SGNS	1	20	SGNR	35	16	< 0.001
Lipocalins: Histamine, serotonin, odorant-binding proteins
	SGNS	909	691	SGNR	347	564	< 0.001
Reprolysin metalloproteases
	SGNS	217	124	SGNR	9	102	< 0.001

Values in bold indicate that there were significantly (Chi-square test) more reads in the respective library than in the corresponding library in the comparison

ISSN: 1756-3305