Table 1 LAMP and nested PCR primer sequences for Pf and non-Pf used in this study
Primer set | Primer | Target species | Sequence (5'-3') |
|---|---|---|---|
Non-Pf CZC-LAMP primer set (7 primers) | Non-Pf.Mt-FIP | Pv, Poc, Pow, Pm | AGGCTGCGATGAGACGACGCCGGGGATAACAGGTTATAGT |
Non-Pf.Mt-BIP | Pv, Poc, Pow, Pm | AGCGTGTATTGTTGCCTTGTACTTAACGCCTGGAGTTCTTTATCTT | |
Non-Pf.Mt-LF | Pv, Poc, Pow, Pm | TGGAGGTGCCAATAGTATATAAAGAT | |
Non-Pf.Mt-LB | Pv, Poc, Pow, Pm | TGTACACACCGCTCGTCAC | |
Pov.Mt-F3 | Pv, Poc, Pow | GGTGATTTTGTGTGCCGTT | |
Pm.Mt-F3 | Pm | ACGGTTATTTTGTGTACCGTT | |
Non-Pf.Mt-B3 | Pv, Poc, Pow, Pm | CGGCTGTTTCCATCTCAACT | |
Pf CZC-LAMP primer set (6 primers) | Pf.newMt-FIP | Pf | CAGTATATTGATATTGCGTGACGACCTTGCAATAAATAATATCTAGCGTGT |
P.f.Mt-BIPa | Pf | AACTCCAGGCGTTAACCTGTAATGATCTTTACGTTAAGGGC | |
Pf.newMt-LF | Pf | GTGTACAAGGCAACAATACACG | |
P.f.Mt-LBa | Pf | GTTGAGATGGAAACAGCCGG | |
Pf.newMt-F3 | Pf | TATTGGCACCTCCATGTCG | |
P.f.Mt-B3a | Pf | AACATTTTTTAGTCCCATGCTAA | |
Non-Pf nested PCR primers | PnfMt-Fout | Pv, Poc, Pow, Pm | TGCTGTCATACATGATGCACTT |
PnfMt-Rout | Pv, Poc, Pow, Pm | ATGTAGTTTCCTCACAGCTTTATTCA | |
PnfMt-Fin | Pv, Poc, Pow, Pm | TGCTGTCATACATGATGCACTT | |
PnfMt-Rin | Pv, Poc, Pow, Pm | ATAACATTTTTTAGTCCCATGCTAGTA |