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Fig. 6 | Parasites & Vectors

Fig. 6

From: Humans from Wuchereria bancrofti endemic area elicit substantial immune response to proteins of the filarial parasite Brugia malayi and its endosymbiont Wolbachia

Fig. 6

Oxidative burst in human PBMCs of bancroftian categories when stimulated with recombinant proteins. Reactive oxygen species (ROS) generation in PBMCs was measured by flow cytometry using DCF-DA after stimulating with recombinant proteins a Bm-TPP; b BmAF-Myo; c Wol Tl IF-1; and d wBm-LigA. Unstimulated PBMCs (US) and stimulated PBMCs (St) data are summarized as mean + standard deviation (n = 5). Presentation of shifting of florescence peak of different proteins in (e) EN group; f MF group; and g CP group. Stimulated groups were compared using Kruskal-Wallis ANOVA and the significance of the differences in mean ranks between groups was assessed by Mann-Whitney U test. * P < 0.05; ** P < 0.01; *** P < 0.001

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