Fig. 6From: Humans from Wuchereria bancrofti endemic area elicit substantial immune response to proteins of the filarial parasite Brugia malayi and its endosymbiont Wolbachia Oxidative burst in human PBMCs of bancroftian categories when stimulated with recombinant proteins. Reactive oxygen species (ROS) generation in PBMCs was measured by flow cytometry using DCF-DA after stimulating with recombinant proteins a Bm-TPP; b BmAF-Myo; c Wol Tl IF-1; and d wBm-LigA. Unstimulated PBMCs (US) and stimulated PBMCs (St) data are summarized as mean + standard deviation (n = 5). Presentation of shifting of florescence peak of different proteins in (e) EN group; f MF group; and g CP group. Stimulated groups were compared using Kruskal-Wallis ANOVA and the significance of the differences in mean ranks between groups was assessed by Mann-Whitney U test. * P < 0.05; ** P < 0.01; *** P < 0.001Back to article page