Humans from Wuchereria bancrofti endemic area elicit substantial immune response to proteins of the filarial parasite Brugia malayi and its endosymbiont Wolbachia

Table 2 Results of ADCC assay against B. malayi L3 using human sera

No.	Groups	% Killing (mean ± SD)
1	Control (L3 + PBMCs)	0
2	NEN (L3 + PBMCs + Pooled NEN sera)	9.7 ± 4.0
3	EN (L3 + PBMCs + Pooled EN sera)	84.1 ± 3.6^a
4	L3 + PBMCs + anti Bm-TPP antibodies depleted Pooled EN sera	30.2 ± 3.0^b
5	L3 + PBMCs + anti BmAF-Myo antibodies depleted Pooled EN sera	51.1 ± 1.9
6	L3 + PBMCs + anti Wol Tl IF-1 antibodies depleted Pooled EN sera	47.6 ± 4.1
7	L3 + PBMCs + anti wBm-LigA antibodies depleted Pooled EN sera	29.4 ± 4.2^b
8	MF (L3 + PBMCs + Pooled MF sera)	31.5 ± 1.6
9	L3 + PBMCs + anti Bm-TPP antibodies depleted Pooled MF sera	21.8 ± 1.6
10	L3 + PBMCs + anti BmAF-Myo antibodies depleted Pooled MF sera	22.9 ± 4.9
11	L3 + PBMCs + anti Wol Tl IF-1 antibodies depleted Pooled MF sera	23.9 ± 3.4
12	L3 + PBMCs + anti wBm-LigA antibodies depleted Pooled MF sera	22.7 ± 3.5
13	CP (L3 + PBMCs + Pooled CP sera)	35.0 ± 3.6
14	L3 + PBMCs + anti Bm-TPP antibodies depleted Pooled CP sera	23.4 ± 1.4
15	L3 + PBMCs + anti BmAF-Myo antibodies depleted Pooled CP sera	27.8 ± 2.9
16	L3 + PBMCs + anti Wol Tl IF-1 antibodies depleted Pooled CP sera	22.1 ± 2.6^b
17	L3 + PBMCs + anti wBm-LigA antibodies depleted Pooled CP sera	24.9 ± 1.8

Data are summarized as Mean ± SD, n = 3. The comparison were done between pooled EN/MF/CP sera and pooled EN/MF/CP sera after depletion of anti Bm-TPP/anti BmAF-Myo/Wol Tl IF-1/anti wBm-LigA antibodies
^a P < 0.05; significant differences between pooled EN sera with pooled MF sera and pooled CP sera
^b P < 0.05; significant differences of % killing in each group after depletions of protein-specific antibodies with respect to undepleted pooled sera

ISSN: 1756-3305