Fig. 9From: RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvaeKnockdown in bmugm gene expression in adult B. malayi male parasites. The relative fold downregulation in mRNA expression of bmugm in B. malayi adult male worms soaked in siRNA1, 2, 1 + 2 was quantified by real time PCR (qRT-PCR) using comparative ΔCT method at different time interval (24, 48, 72, 96, 120 h), β-tubulin was the endogenous control housekeeping gene. The above results depict the mean ± standard deviation (SD) derived from three separate experiments carried out under similar conditions. The gel images depicting the target (bmugm) and endogenous control (β-tubulin) gene expression status at different time points are shown on top (a-e). ***P < 0.001 (very highly significant)Back to article page