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Table 4 Effect of bmugm gene silencing on viability of adult B. malayi male parasites was assessed by motility scoring and MTT reduction assay

From: RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae

Time (h) Motility score (male worms)a % inhibition in MTT reduction (mean ± SD)
MC SCR siRNA 1 siRNA 2 siRNA 1 + 2 NC siRNA 1 siRNA 2 siRNA 1 + 2
24 5+ 5+ 2+ 2+ 2+ 11.6 ± 1.98 69.1 ± 9.93** 69.8 ± 2.90** 69.9 ± 2.13**
48 4+ 4+ 1+ 1+ 1+ 5.9 ± 2.95 60.7 ± 10.47** 77.2 ± 8.97*** 84.9 ± 11.95***
72 4+ 4+ 1+ 1+ 1+ 7.1 ± 3.68 80.5 ± 9.27*** 85.70 ± 8.50*** 92.7 ± 0.88***
96 3/4+ 3/4+ 1+ 1+ 1+ 6.2 ± 2.35 90.8 ± 3.60*** 87.3 ± 9.60*** 90.6 ± 9.53***
120 3/4+ 3/4+ 1+ 1+ 1+ 7.9 ± 4.13 85.2 ± 2.75*** 83.5 ± 9.85*** 90.9 ± 1.83***
  1. Abbreviations: NC negative control (scrambled siRNA); MC medium control, MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide); SCR negative control (scrambled siRNA); SD standard deviation
  2. **P < 0.01; ***P < 0.001
  3. a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction