RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae

Table 4 Effect of bmugm gene silencing on viability of adult B. malayi male parasites was assessed by motility scoring and MTT reduction assay

Time (h)	Motility score (male worms)^a					% inhibition in MTT reduction (mean ± SD)
Time (h)	MC	SCR	siRNA 1	siRNA 2	siRNA 1 + 2	NC	siRNA 1	siRNA 2	siRNA 1 + 2
24	5+	5+	2+	2+	2+	11.6 ± 1.98	69.1 ± 9.93**	69.8 ± 2.90**	69.9 ± 2.13**
48	4+	4+	1+	1+	1+	5.9 ± 2.95	60.7 ± 10.47**	77.2 ± 8.97***	84.9 ± 11.95***
72	4+	4+	1+	1+	1+	7.1 ± 3.68	80.5 ± 9.27***	85.70 ± 8.50***	92.7 ± 0.88***
96	3/4+	3/4+	1+	1+	1+	6.2 ± 2.35	90.8 ± 3.60***	87.3 ± 9.60***	90.6 ± 9.53***
120	3/4+	3/4+	1+	1+	1+	7.9 ± 4.13	85.2 ± 2.75***	83.5 ± 9.85***	90.9 ± 1.83***

Abbreviations: NC negative control (scrambled siRNA); MC medium control, MTT (3-(4, 5 dimethylthiazol-2-yl)-2, 5 diphenyltetrazolium bromide); SCR negative control (scrambled siRNA); SD standard deviation
**P < 0.01; ***P < 0.001
^a5, 0% motility reduction; 4, 1–25% reduction; 3, 26–49% reduction; 2, 50–74% reduction; 1, 75–99% reduction; D (death), 100% reduction

ISSN: 1756-3305